PJB-2023-224
Heat shock treatment enhances the ability of Chinese yam to resist Curvularia lunata infection
Shuo Wang, Peiyan Wang, Lanting Qi, Yuguang Zheng, Xianyun Wang, Yuping Yan, Ruojia Zhu and Zhao Liu
Abstract
This experiment explored the effects of different heat-shock temperatures on the disease resistance of yam, aiming to enhance resistance to Curvularia lunata infection and reduce pesticide usage without compromising yam quality. Yam seedlings were subjected to heat treatments for 1.5 hours before inoculating them with Curvularia lunata. Changes in yam leaves were observed using Diaminobenzidine and lactophenol-trypan blue staining methods. The expression of heat-shock genes and disease-resistance genes PTI5 and PR1 in yam plants was detected by qPCR. The Allantoin of yams was measured by HPLC, and the polysaccharide content was determined by the phenol-sulfuric acid method. The results showed that the leaves of yam subjected to the 45℃ heat treatment exhibited minimal damage and heightened resistance to Curvularia lunata. Conversely, leaves from non-treated yams showed significant damage. qPCR analysis confirmed that 45℃ heat treatment significantly upregulated heat shock and anti-fungal gene expression, aligning with phenotypic observations. Furthermore, urea and polysaccharide contents were increased in yams after the 45℃ heat treatment. The study demonstrated a cross-response mechanism between yam heat-shock and disease resistance genes, offering an effective solution to fungal diseases and pesticide issues in yams, potentially improving crop quality. These findings may serve as a reference for yam disease resistance and breeding and could apply to other crops for developing eco-friendly disease resistance strategies
To Cite this article:
Wang, S., P. Wang, L. Qi, Y. Zheng, X. Wang, Y. Yan, R. Zhu and Z. Liu. 2025. Heat shock treatment enhances the ability of Chinese yam to resist Curvularia lunata infection. Pak. J. Bot., 57(1): DOI: http://dx.doi.org/10.30848/PJB2025-1(26)
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