PJB-2023-233
Optimization of Embryo Rescue Technique for Development of Hybrid Plants in Stenospermic Grapes
QANDEELA NIGAR
Abstract
Development of seedless cultivars is one of the major objectives in grapevine breeding. Grapes being stenospermic crop, aborts its embryo before development so traditional breeding method results in production of seedless cultivars with low frequency. Embryo rescue is the only promising way for the development of seedless cultivars. The study was conducted to optimize the best sampling time and protocol for efficient embryo rescue in grapes. Ovules form immature berries collected at different days after pollination (20DAP, 30DAP, 40DAP and 50DAP) were inoculated on Nitsch and Nitsch (NN) medium having different concentrations of Benzyl aminopurine (0.1, 0.2 and 0.5 mg/L) and Activated Charcoal (1.5, 2 and 2.5 g/L). Parameters like maximum ovule growth, percentage of enlarged ovule, percentage of collapsed ovule, callus formation percentage and embryo germination percentage were recorded. Results revealed that berries collected at 40 DAP performed best for all parameters. Regarding treatments best results were obtained by ovules inoculated on NN medium in combination with 0.5mg/L BAP and 2g/L AC. So, it can be concluded that best sampling time and treatments play an important role in efficient embryo rescue in grapes. The standardized protocol is recommended for efficient embryo rescue for the development of seedless hybrids.
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