PJB-2023-340
Phenotypic, biochemical and molecular diversity and divergence in Brassica accessions representing different species
Gohar Zaman, Tamana Bakht, Muhammad Rameez Khan, Iqbal Munir, Hafiz Munir Ahmad, Aamir Iqbal, Safi Ullah, Muhammad Arif and Sajid Ali
Abstract
Genetic improvement in Brassica is crucial for the production of sufficient edible oil, which requires exploitation of diverse genetic resources. The present study assessed divergence and diversity of 24 Brassica accessions belonging to different species through molecular genotyping, biochemical characterization and field testing. Considering yield contributing parameters, number of siliquas main raceme-1 ranged from 39.3 (local genotype) to 48.7 siliqas (IBGE-2); number of primary branches ranged from 3.3 (wester-209) to 6.0 (p118 and Altex 232); number of siliquas plant-1 ranged from 173 (Torch-237) to 409 (IBGE-4); seeds siliqua-1 ranged from 13 (IBGE-4) to 21 (p-118); yield ranged from 560 g (Mun-1) to 1462 g (IBGE-4). Variability was also observed for oil content (%), which ranged from 47 (Zahoor-15 and IBGE-1) to 53 (Mun-1 and Crusher-212), while the protein content was overall in higher content, with the highest value of 24% (Zahoor-15). Molecular genotyping with a set of 16 polymorphic loci revealed the maximum number of loci (5) for OPC-05, followed by OPC-09 and OPB-04 (4). The maximum gene diversity (0.497) was recorded for loci OPB-04-L3, while the minimum (0.080) was for locus OPC-05-L5. The neighbor-joining tree showed that accessions representing B. juncea were dispersed among various genotypes. In network analysis, two clusters were identified. Cluster I consisted of B. compestres while cluster II comprised of B. juncea with similarity to other B. napus crossed genotypes. The divergence and diversity observed in the present study should be useful for genetic improvement
To Cite this article:
Zaman, G., T. Bakht, M.R. Khan, I. Munir, H.M. Ahmad, A. Iqbal, S. Ullah, M. Arif and S. Ali. 2025. Phenotypic, biochemical and molecular diversity and divergence in Brassica accessions representing different species. Pak. J. Bot., 57(2): DOI: http://dx.doi.org/10.30848/PJB2025-2(25)
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