PJB-2023-342
Stabilization of disturbances in membrane photochemical reactions in wheat seedlings under cold stress by natural exogenous saponins
Rena Ganiyeva, Sevil Dadashova, Dolores Hasanova, Marina Zarkua, Samira Chyragova and Ralphreed Hasanov
Abstract
The chloroplast membrane is under the constant effect of external factors (temperature, light, nutrition, etc.) resulting in certain structural changes. Low temperature is known to contribute to a decrease in the fluidity of the lipid phase of the membrane. Metabolites of cell lipid metabolism, in turn, can facilitate the mobility of protein components. Modifying these conditions, we used the incubation of Triticum aestivum L. seedlings at a low temperature (4°C) for various periods as a hardening effect of membrane lipids In vivo. Adding a fraction of saponins – natural detergents, isolated from the medicinal plant Glycyrrhiza glabra into the nutrient solution provoked a change in the state of the thylakoid membrane. Under these conditions, the behavior of the change in the delayed chlorophyll a fluorescence and pigments activity in the light-harvesting antenna were studied In vivo using the absorption spectra. It was shown that in wheat seedlings in the absence of saponins, after 4 hours of cold stress, the activity of the electron transport chain in the PS II decreases, which is associated with the blocking of electron transfer in the quinone acceptor site. Longer cold stress contributed to the restoration of these changes. The maximum change in the compositions of photosynthetic pigments occurred after 48 hours of cold stress. The introduction of saponins into the nutrient solution prevented changes in the reactions of the electron transport chain of PS II, which was correlated with the restoration of the pigment pool in the light-harvesting antenna. The latter is probably associated with an adaptive increase in membrane stability. The corrective effect of the saponin fraction is possible due to the restoration of lipid components of the thylakoid membrane, and the transition of the membrane from a quasicrystalline to a liquid state. Restoration of membrane fluidity stimulates the function of membrane proteins - electron carriers in the PSII electron transport chain and stabilizes the light-harvesting pigment complex
To Cite this article:
Ganiyeva, R., S. Dadashova, D. Hasanova, M. Zarkua, S. Chyragova and R. Hasanov. 2024. Stabilization of disturbances in membrane photochemical reactions in wheat seedlings under cold stress by natural exogenous saponins. Pak. J. Bot., 56(6): DOI: http://dx.doi.org/10.30848/PJB2024-6(22)
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