PJB-2023-406
Correlation of polyphenolic content, antioxidant and ecological factors in Chinese seabuckthorn (Hippophae rhamnoides L. subsp.
Hui Su
Abstract
Abstract: Chinese seabuckthorn (Hippophae rhamnoides L. subsp. sinensis Rousi) is a pioneer plantation species and an important resource plant often grown in the temperate zone at 800-3600 m above sea level, and its leaves are rich in polyphenols. It is important to clarify the content of polyphenols in the leaves of Chinese seabuckthorn and their relationship with ecological factors for the collection and utilization of seabuckthorn resources. Thirty-four natural populations of Chinese seabuckthorn distributed in Gansu, Hebei, Inner Mongolia, Ningxia Hui Autonomous Region, Qinghai, Shanxi, and Shaanxi provinces were studied. The contents of polyphenols and their antioxidant activity in Chinese seabuckthorn leaves were determined by field investigation and laboratory analysis. The relationship between polyphenol content, antioxidant and 16 ecological factors (Latitude, longitude, altitude, average annual rainfall, rainfall in growing season, average monthly rainfall in growing season, rainfall in dry season, average monthly rainfall in dry season, average annual temperature, average annual maximum temperature, average annual minimum temperature, average annual radiation, temperature in growing season, temperature in dry season, steam pressure, wind speed) in leaves of Chinese seabuckthorn was analyzed by correlation network diagram analysis. The results showed that: The Chinese seabuckthorn polyphenol content ranged from 915.36 mg/g to 2134.91 mg/g, the flavonoid content ranged from 196.26 mg/g to 657.80 mg/g, and the flavanol content ranged from 86.50 mg/g to 501.01 mg/g. The scavenging capacity of Chinese seabuckthorn leaf polyphenolic extracts on DPPH free radicals, Cu2+ reduction ability, and metal ion chelation ability were significantly different (P<0.05). The correlation analysis revealed that the polyphenolic content and antioxidant capacity of Chinese Seabuckthorn from different collection sites were significantly and positively correlated (P<0.05). The correlation network diagram analysis showed that Chinese seabuckthorn polyphenols, DPPH scavenging ability, and metal chelating ability were positively correlated with altitude. And the 34 natural populations were divided into four groups by cluster analysis;Through comprehensive evaluation of the affiliation function, the 10 best-performing species sources were finally selected from 34 natural populations of Chinese seabuckthorn, which could be exploited as natural antioxidants of the plant.
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