PJB-2023-507
16S-rRNA BASED MOLECULAR CHARACTERIZATION OF PLANT PATHOGENIC BACTERIAL SPECIES ASSOCIATED WITH CITRUS, PAKISTAN
Iram Zaheer
Abstract
The percentage of losses attributable to citrus disease brought on by bacterial infections has increased worldwide. However, the mature citrus trees in Pakistan exhibit diseases before and after harvesting. However, the exact disease agents have not yet been discovered. The morphological and molecular characterization of the bacterial pathogens in the current study was complex, as was the evaluation of citrus disease's prevalence, incidence, and severity. The bacterial pathogens were isolated from infected obtained samples using tissue culture and streaking procedures in the first phase of the study during the month of December. They were then identified based on their morphology. Second, the isolated strains were characterized using the primers 27F (5-CTTCAACTCAAACGCCGGA-3) and 1492R (3-CATCGGCTGTTCGGGAG-5), which were used for the identification of the 16S rDNA region. Thirdly, fresh leaves of two citrus varieties (Succri and Moro blood) were used to investigate the pathogenicity of isolated bacterial strains. The pathotypes of Xanthomonas sp. caused the most severe symptoms. This is the first study we are aware of on citrus pathogens in Khanpur. This study shows the molecular-based association of bacterial pathogens with citrus diseases, which helps to provide recommendations on genetic testing and develop disease management programs, as citrus fruits significantly contribute to household income and foreign exchange in Pakistan.
Keywords Citrus Fruits, Canker, Xanthomonas pathotypes, Yield loss, Epidemiology
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