PJB-2023-529
In vitro Antibacterial efficacy against folliculitis and dermatitis bacteria, toxicity study, and Phytochemical profiling of Ficus carica Fruit&
Shahlla Umbreen
Abstract
Aim: The aim of this study is to find the antibacterial activity of Ficus carica fruit against bacteria isolated from dermatitis and folliculitis and the screening of its phytochemical profile. Thus provide the basis of therapeutic use of this plant against diseases of skin and hair follicles. In this way, the alarming situation of antibacterial resistance can be battled by using secondary metabolites of plants.
Material and Methods: Ficus carica fruit were collected, identified and authenticated. After washing, slicing, drying and grinding, the extracts were made in different solvents like ethanol, methanol, petroleum ether and acetone by maintain the plant powder and solvent ratio of 1:12. Samples were taken from dermatitis and folliculitis patients by pre moistened Z stroke manner and proceeded in laboratory for chemical characterization and isolation of bacteria. The isolated bacteria were subjected to extracts by disk diffusion and well diffusion method to analyze the Zone of inhibition, MIC and MBC. The extract type with best antibacterial activity were continued further for qualitative phytochemical screening, GC-MS and HPLC. In vitro study was done for evaluating the toxicity and safety dose.
Results: The maximum yield extract was obtained from Methanol. Gram positive strains Staphylococcus aureus and Staphylococcus epidermidis however, in Gram negative strains Escherichia coli, Pseudomonas aeruginosa and Klebsiella pneumonia were isolated. The extracts showed dose response ZOI and FCFM were found to be greatly effective with 15mm to 16mm ZOI for both Gram positive and negative. While FCFA and FCFE was also effective with 11mm zone. FCFM were found to be the rich source of carbohydrate, alkaloid, flavonoids, tannins etc. On GCMS of FCFM the compound identified included Bis(2-ethylhexyl) phthalate, Oleic Acid, 1,4-Benzenedicarboxylic acid, bis[4-(ethenyloxy)butyl] ester, γ-Sitosterol, phytol and many others. While on HPLC, quercetin, gallic acid, chlorogenic acid, sinapic and many other phenolic acids were identified. FCFM was also found safe while performing in vitro study on rats.
Key words: ZOI-Zone of Inhibition, MIC-Minimum Inhibitory Concentration, MBC-Minimum Bactericidal Concentration, FCFM-Ficus carica Fruit Methanol, FCFA- Ficus carica Fruit Acetone, FCFE- Ficus carica Fruit Ethanol, GCMS- Gas Chromatography Mass Spectrometry, HPLC-High Performance Liquid Chromatography.
To Cite this article:
Download
