PJB-2023-572
IDENTIFYING ANGPT-4 AS A POTENTIAL GENE IN PROGRESSION OF HEPATOCELLULAR CARCINOMA USING BIOINFORMATICS TOOLS AND ANALYSIS
Akash K
Abstract
Hepatocellular carcinoma (HCC) is a deadly malignant and it is yet to ascertain the mechanism of HCC initiation, development, and progression, in order to explore the differently expressed genes that contribute to HCC progression hence, we downloaded the gene profile GSE33006 from the GEO database in NCBI with 3 HCC tumor-infected samples and 3 normal liver samples to examine and find the differently expressed genes. To accomplish that the following bioinformatics tools have been incorporated, so the downloaded data was analyzed with set-parameters Gene Ontology (GO) analysis, Protein-protein Interaction (PPI) network analysis, Target-factor (TF) gene Interactions, and using Kyoto Encyclopedia of Genes and Genomes (KEGG) for pathway analysis and STRING database was also used for enrichment and validation of genes. As a result, a total of 896 genes were seen as Up-regulated genes and 368 genes were down-regulated. From the analysis among the Up-regulated genes, Angiopoietin 4 (ANGPT-4) was identified as one of the feasible candidate genes. The expression of ANGPT-4 shows results similar to past conducted research proving similarities between both data. This study provides insight into the prognosis of HCC and the possible gene ANGPT-4 along with the HIF-1α signaling pathway that is responsible for the progression of Hepatocellular carcinoma.
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