PJB-2023-574
Cloning of RhBEE1-like gene and the effect on petal senescence in rose
Wenyu Wang, Tingting Du, Siwen Han, Jingjing Zhang, Zhe Qin and Haixia Pei
Abstract
Rosa hybrida is one of the most important cut flowers in the world, the premature senescence of its petals seriously affects its quality and economic value. In order to explore the senescence mechanism of rose petals and provide gene reserve for the creation of anti-senescence rose varieties through molecular breeding and other means, a bHLH transcription factor (TF) was screened based on our previous transcriptome data. bHLH TFs have been shown to play important roles in the plant growth and development, and in response to stress response. In this study, we investigated the function of bHLH TF in petal senescence of cut rose ‘Carola’. We cloned a gene named RhBEE1-like belonging to bHLH TF family. Quantitative RT-PCR (qRT-PCR) showed that the expression level of RhBEE1-like increased significantly with the opening of flowers in rose and was significantly induced by ethylene. Transient overexpression of RhBEE1-like in tobacco mesophyll cells showed that the GFP signal of RhBEE1-like coincided with the nuclear signal of DAPI through confocal microscope, indicating that RhBEE1-like was located in the nucleus. Silencing RhBEE1-like in rose petals by virus-induced gene silencing (VIGS) can accelerate the senescence of rose petals, and at the same time, the expression level of senescence marker gene RhSAG12, the ion leakage rate and fading rate of rose petals were significantly increased. To summarize, our study showed that RhBEE1-like has antagonistic effect on petal senescence in rose
To Cite this article:
Wang, W., T. Du, S. Han, J. Zhang, Z. Qin and H. Pei. 2024. Cloning of RhBEE1-like gene and the effect on petal senescence in rose. Pak. J. Bot., 56(6): DOI: http://dx.doi.org/10.30848/PJB2024-6(25)
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