PJB-2024-1
INVITRO PROPAGATION AND ACCLIMATIZATION OF KINNOW MANDARIN (CITRUS RETICULATA BLANCO) UNDER HOT ARID CLIMATIC CONDITIONS.
Syeda Kahkashan Kazmi
Abstract
The Citrus genus is inhabitant of the tropical and subtropical regions of Pakistan, India and southern China, New Caledonia and northern Australia. In this study direct regeneration of Kinnow mandarin shoots was performed from internodal stem segments and acclimatization under arid hot climatic conditions. MT medium supplemented with 0.5 mg/L malt extract and 50 g/L sucrose along with growth hormones BAP and Kinetin in thirty-six media formulations were tested. At 0.05 level of significance MT1 media containing 0.5mg/L BAP induced slightly significant highest mean number of shoots. Whereas, on MT45 media containing 3mg/L BAP and 1.5mg/L Kinetin the regenerated plantlets attained the non-significant highest mean length of shoots. It was observed that MTR4 media containing 0.4mg/L IBA and 0.1mg/L NAA and 0.5mg/L activated charcoal induced non-significant highest mean length of roots. Highly significant highest numbers of roots were observed on MTR10 media containing 1mg/L IBA and 0.1mg/L NAA along with 0.5mg/L activated charcoal the rooted plants were given heat shock under invitro condition and then acclimatized in hot arid climatic conditions. This study is supportive in efficient micropropagation and acclimatization of Kinnow mandarin Citrus reticulata Blanco.
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