PJB-2024-51
SCREENING AND IDENTIFICATION OF ANTAGONISTIC FUNGI OF PEANUT WEB&
Yufan Wang
Abstract
Peanut web blotch is one of the fast-spreading and heavily infested peanut leaf spot diseases, which mainly affects leaves, but also petioles and stalks. In this study, we screened fungi from soil with significant inhibitory effect on peanut web blotch fungi, and used the growth rate method to re-screen, screened biocontrol strains with better inhibitory effect, and identified them. In this study, we also did the fungal inhibition spectrum test, and used the fermentation broth for leaf in vitro culture and greenhouse potting prevention effect test. The results showed that strain F11 performed well, with an initial sieve inhibition band of 7.56 mm and a resieve inhibition rate of 63.95%. After morphological identification and molecular biology, F11 strain was identified as Talaromyces sayulitensis. through the inhibition spectrum test, it was found that the sterile fermentation broth of F11 had a certain inhibitory effect on other pathogens as well. The inhibition rates of Exserohilum turcicum and Colletotrichum orbiculare reached 83.02% and 81.54% against the corn big spot pathogen and cucumber anthracnose pathogen, respectively. The fermentation solution of strain F11 was used for the raw control effect test, and the research results showed that the control effect could reach 87.65%.
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