PJB-2024-86
COMPARATIVE EVALUATION OF ANTIBACTERIAL EFFICACY OF AGNPS OF PIPAR NIGRUM AND 95% ETHANOLIC LEAF EXTRACT OF ALOE VERA L. AGAINST STAPHYLOCOCCUS AUREUS AND E.COLI
Anum Ijaz
Abstract
Medicinal plants are being looked at as potential new sources for manufacturing antibiotic-like compounds. Using two methods, Disc diffusion and Agar well method, antibacterial activity of Aloe vera L. ethanolic extract activity and Silver nanoparticles (AgNps) of Pipar nigrum was evaluated. Four different concentrations of silver nanoparticles and Aloe vera extract against two bacterial strains, Escherishia. coli and staphlococuss. aureus at level of (5.0, 10, 15, and 20 mg/ml) respectively was used.. Aloe vera extract and Silver Nano particles exposed a maximum region of inhibition against gram negative bacteria than gram positive when tested via the disc diffusion method. The inhibition value of plant and nanoparticles with Stapphylococcus aureus 0.123 nm and 0.549 nm, respectively, when different concentrations (5-20 mg/ml) were used, whereas the lowest MIC value was 0.432 nm and 0.321 nm for Aloe vera and silver nanoparticles contrary to E.coli at a concentration level of 10 mg/ml. Flavonoids, terpenoids, tannins, polyphenol, and saponins were found in both Aloe vera and Silver nanoparticles, while saponins were not found in Silver Nanoparticles.
Key words: Aloe vera, Silver nanoparticles, Pipar nigrum and Antibiotics.
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