PJB-2024-132
Suleiman Hussain Shah
Abstract
This study explored the anti-asthmatic potential of bark of Cassia fistula using Mus musculus as model organism. The mice were treated with Ovalbumin on day one and the 14th day and then herbal extract doses were given from day 15th to 26th to all groups except normal and asthmatic control, and oral inhalation of Ovalbumin from day 27th to 29th to all treatment groups except control. Mice were divided into six treatment groups i.e., Normal, C. fistula (10mg/ml), C. fistula (15mg/ml), C. fistula (20mg/ml), Dex (3mg/ml), Asthmatic (Control) and dose administered orally. Blood serum analysis showed liver enzymes (alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase) and bilirubin, total proteins, albumin and globulin tests, the liver function test showed lower values in all treatment groups except asthmatic control. In asthmatic control, hematological parameters of red blood cells (MCV, HCT, MCH, Hemoglobin, and MCHC) and platelets declined, whereas white blood cells (Neutrophils, Lymphocytes, Monocytes and Eosinophils) were increased. However, hematological parameters showed restoration in all C. fistula groups and DEX group. In all treated groups, total cells (Neutrophils, Lymphocytes, Monocytes and Eosinophils) of Bronchoalveolar Lavage Fluid (BALF) also decreased as compared to asthmatic control. ELISA results confirmed that the levels of Interleukins such as IL-4, IL-5, IL-13, IL-17A, IgE decreased in treated groups as compared to asthmatic control. However, over stimulation IL-10 was observed in treated groups than asthmatic control. Real-time PCR results confirmed that the relative mRNA expression of cytokines (IL-4, IL-5, IL-13, IL-17A) reduce in all treated groups as compared to asthmatic control. Moreover, over stimulation IL-10 (anti-cytokine) was seen in all treated groups as compared to asthmatic control. Histological studies revealed that airways constrictions (epithelium thickness, smooth muscle thickness and bronchiole) were reduced in all treated groups except asthmatic control. The study concludes that C. fistula has shown potential to reduce asthma, improved hematological parameters positively and suppresses and overshoots the cytokines and anti-cytokine, respectively. C. fistula reduces airways remodeling in asthmatic mice.
To Cite this article:
Download
