PJB-2024-196
Optimization of fermentation medium and fermentation conditions of biocontrol strain F11
Yi Zhang, Yufan Wang, Wenxing Li, Lingyue Zhu, Xiaoxu Sun, Kangsheng Tian, Peiran Wang and Xiaomei Wang
Abstract
Peanut web blotch is one of the more prevalent and rapidly spreading diseases of peanut. In this study, soil samples were collected from different crops and vegetable rhizosphere soils in different regions, and biocontrol fungus F11 was isolated and screened. The strain had a good inhibitory effect on Didymella arachidicola, and it was identified as Talaromyces sayulitensis by morphological identification and molecular biology. The fermentation medium and fermentation conditions were optimized by single factor test, orthogonal test and response surface test. The results showed that the optimum fermentation medium was corn gluten meal 24.33 g·L-1, diammonium hydrogenphosphate 2.56 g·L-1, and CaCO3 1.52 g·L-1. The model was established by Design Expert software, and the bacteriostatic rate under the above conditions was predicted to be 87.92%. The actual inhibition rate obtained after verification was 87.65%, which was close to the predicted value. Compared with 63.95% before optimization, it increased by 23.70%. The optimum fermentation conditions were as follows : liquid volume was 125 mL, temperature was 30°C, and fermentation time was 7 d.
To Cite this article:
Zhang, Y., Y. Wang, W. Li, L. Zhu, X. Sun, K. Tian, P. Wang and X. Wang. 2025. Optimization of fermentation medium and fermentation conditions of biocontrol strain F11. Pak. J. Bot., 57(6): DOI: http://dx.doi.org/10.30848/PJB2025-6(3)
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