PJB-2024-206
INDUCTION ROLE OF TANNIC ACID IN PRODUCTION OF TANNASE BY SUBMERGE FERMENTATION FROM ASPERGILLUS SPECIES
TANZEELA REHMAN
Abstract
Tannin acyl hydrolase commonly called “tannase” is an important industrial enzyme mostly produced from Aspergillus species. Agro wastes are the best source of tannic acid. In this study, Aspergillus flavus was used to produce the tannase by using different plant leaves under submerged fermentation. The leaves of guava (Psidium guajava), pomegranate (Punica granatum), tea (Camellia sinensis), grapes (Vitis vinifera) and kekeer (Acacia nilotica) were used as a substrate. Maximum tannase production was found on leaves of Vitis vinifera after 72 h incubation at 30ºC (pH 6.5). The Colorimetric assay method was used for the measurement of enzyme activity. Purification of tannase was completed in two steps 1) ammonium sulfate precipitation and 2) gel filtration chromatography (Sephadex G-200). The SDS-PAGE (10%) indicated the presence of 33 kDa protein band of tannase. This procedure was economical to produce important industrial enzyme (tannase) by utilizing the cheap source of guava leaves. This study revealed that the plant waste of guava produced the maximum tannase enzyme in an economical way.
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