PJB-2024-223
Barcoding of Chenopodium murale L. with new record to Egyptian flora
Amany S. Abdo, Wafaa M. Amer, Najla A. Al-Shaye and Walaa A. Hassan
Abstract
Chenopodium L. is a cosmopolitan genus (family: Amaranthaceae). Hybridization and phenotypic plasticity of its species, complicate the species delimitation. The presence of observed notable phenotypic plasticity among the C. murale populations and the newly recorded (suspected C. hybridum), makes it challenging to identify these taxa's taxonomic identity. The morphological investigations revealed distinctive features at inter-specific level for C. murale populations mainly in stem color, leaf shape, margin dentation, number of stamens and tepals, and the percentages of both hermaphrodite flowers and the 5-staminode flowers. The morphological correlation between distinguished Forms; ranges between 0.070-0.518. While the infra-specific correlation between the C. murale forms and the suspected C. hybridum ranges from 0.033-0.340. RbcL and matK barcoding were applied to confirm the taxonomic identity as well as, their phylogenetic affinities of the studied taxa. The retrieved phylogenetic affinity between C. murale forms showed very low genetic divergence to the C. hybridum. The sequences of the confirmed C. murale forms and the new record to Egyptian flora C. hybridum were deposited in the world database under the accession numbers OR373113, OR394977, OR394978, OR394979, OR405949, OR405950, OR405951, and OR405952 for the C. murale and OR394980 and OR405953 for the C. hybridum. We investigated the inter-specific relationships among the C. murale forms, but the intra-specific relationships between C. murale and C. hybridum required further investigation to explore and fully understand the origin of this new record
To Cite this article:
Amany, S.A., W.M. Amer, N.A. Al-Shaye and W.A. Hassan. 2025. Barcoding of Chenopodium murale L. with new record to Egyptian flora. Pak. J. Bot., 57(2): DOI: http://dx.doi.org/10.30848/PJB2025-2(20)
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