PJB-2024-227
PHYTOCHEMICAL PROFILING OF Solanum sisymbriifolium ETHANOL EXTRACT USING GC-MS AND FT-IR ANALYSIS AND EVALUATION OF ITS ANTIBACTERIAL AND ANTICANCER POTENTIAL
GAMAL AWAD El-SHABOURY
Abstract
Solanum sisymbriifolium, considered an invasive plant, is used as traditional medicine to treat both human and veterinary diseases. Our study aimed to identify the active phytochemicals from the ethanol extract of S. sisymbriifolium aerial parts using gas chromatography-mass spectroscopy (GC-MS) and Fourier transform infrared spectroscopy (FT-IR), to investigate its potential as an anticancer agent against hepatocellular and breast cancer (HepG2 and MCF-7) and to examine its antibacterial potential. The presence of phenols, alcohols, ethers, alkynes, and amides in the plant extract was confirmed by FT-IR analysis, among which phenolic compounds have antibacterial and anticancer potential. Furthermore, GC-MS analysis yielded 48 phytochemical substances with varying retention times, including n-Pentadecanol with a molecular weight of 228.414 g/mol, which has anticancer potential. On the other hand, the cytotoxic assay revealed that the extract tested against tumor cell lines (MCF-7 and HepG2) showed variable cytotoxic activity, with the most cytotoxic effect on MCF-7 cells with IC50 of 25.3 ± 2.8µg and a moderate cytotoxic profile on HepG2 cells with IC50 of 41.2 ± 6.1 µg. Examined Gram-negative and Gram-positive bacteria were sensitive to the extract. Our findings suggest that S. sisymbriifolium aerial parts ethanol extract could be used as an antibacterial agent and a natural therapeutic candidate for hepatocellular and breast cancer.
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