PJB-2024-274
IDENTIFICATION AND EXPRESSION ANALYSIS OF THE MELON GLK TRANSCRIPTION FACTOR FAMILY UNDER FUSARIUM WILT STRESS
LING ZHENG
Abstract
Abstract: GOLDEN2-LIKE (GLK or G2-like) transcription factors play a crucial role in regulating the growth and development of plant chloroplasts, plant senescence, and fruit quality. This study identified members of the melon GLK gene family using bioinformatics methods and analyzed them in terms of phylogenetic relationships, gene structure, conserved motifs, synteny, chromosomal localization, and expression patterns. The study also assessed the specific expression of this gene family under Fusarium wilt stress. Results indicated that there are 43 genes encoding GLK transcription factors in the melon genome, which can be classified into five groups unevenly distributed across 13 chromosomes. Expression levels of the melon GLK gene family are significantly higher in leaves compared to other tissues. Light-responsive elements are abundant among the cis-acting elements, and jasmonate and salicylic acid-responsive elements related to stress responses are closely associated with plant stress resistance. qRT-PCR results showed that several CmGLK genes exhibited increased expression following Fusarium infection, particularly at 24 and 48 hours post-inoculation, with increases of several to more than tenfold. CmGLK33 showed a significant increase in expression 24 hours post-infection, while CmGLK10, CmGLK12 and CmGLK20 peaked at 48 hours. CmGLK19 showed a significant increase at both 24 and 48 hours. This study identified and analyzed the GLK gene family in melon, providing a basis for further exploration of the functions of the CmGLK gene family.
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