PJB-2024-291
ESTABLISHMENT OF TISSUE CULTURE REGENERATION SYSTEM OF G
Wan-yun Wu
Abstract
In order to establish a simple and efficient tissue culture regeneration system for Gymnotheca chinensis Decne., the tender stems and terminal buds of G. chinensis cultivated in the wild as experimental materials to explore the optimal explant, sterilization conditions of explant, medium for adventitious bud induction, and medium for rooting and subculture in the process of plant tissue culture. The results showed that the explants were soaked in 0.5 mg/L penicillin sodium+0.5 mg/L streptomycin mixed solution for 3 h+75% ethanol treatment for 30 s+0.1% HgCl2 sterilization for 20 min+0.1% HgCl2 sterilization for 15 min, and then inoculated in the culture medium. The top bud as the explant had the best effect, with the pollution rate of 22.2% and the browning rate of 33.3%; MS+2 mg/L 6-BA+0.5 mg/L NAA+0.2 g/L activated carbon was the best primary medium with 96.7% bud; MS+1mg/L NAA+0.5 mg/L IBA+0.2 g/L activated carbon as rooting medium, the rooting rate was 66.7%; MS+1.0 mg/L NAA+1.0 mg/L IBA+0.2 g/L activated carbon was the best subculture medium, and the rooting rate was 88.9%. This stable tissue culture and regeneration system of G. chinensis, which will provide technical reference for artificial breeding and biological experiments of G. chinensis.
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