PJB-2024-305
COMPARISON OF NATURAL AND SYNTHETIC PRESERVATIVE TO COMBAT FISH (NILE TILAPIA) SPOILAGE MICRO ORGANISMS
Akash Sahar
Abstract
The wild melon species also known as colocynth produces cantaloupe-sized fruits with a bitter flavor and seeds that are high in protein and oil. It is a member of the Cucurbitaceae family of melon species. The current study intends to investigate antibacterial properties of C. colocynthis on gram-negative bacteria E.coli & gram positive bacteria S.aureus. Natural antimicrobials derived from plants, animals, and microbes have the potential to replace chemical antimicrobials. Utilizing UV spectrometery, the chemical characterization of seeds was accomplished. MIC determination was done by using ELISA technique. Nile tilapia fish species was used for this experiment to determine antibacterial potential of C. colocynthis. The result of this study shows that the synthetic preservatives have negative effects on fish and also don't encourage fish growth, thus they need to be replaced with natural preservatives that don't negatively impact fish and also encourage fish growth.
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