PJB-2024-307
Effects of nitrogen-fixing bacteria on Physiological Indices, Antioxidant Enzyme activities and Corresponding Gene Relative Expression differences in Fritillaria taipaiensis P. Y. Li
Mingyan Ye
Abstract
The present investigation was designed to explore the ramifications of inoculating Fritillaria taipaiensis P. Y. Li with nitrogen-fixing bacteria, focusing on variations in physiological indices, antioxidant enzyme activities, and corresponding gene expression levels. A room-temperature pot experiment was meticulously carried out at Hongchi Dam, Wuxi County, Chongqing City, during the 2022-2023 period. The experimental design included the inoculation of eight treatment groups. The goal was to investigate the effects of these bacterial treatments on physiological indices, photosynthetic parameters, photosynthetic pigment content, and both antioxidant enzyme activities and gene expression within the leaves of F. taipaiensis P. Y. Li. The findings revealed that in comparison to the CK group, the content of malondialdehyde (MDA) and stomatal limiting value (LS) in the treated leaves of F. taipaiensis P. Y. Li was markedly diminished (P<0.05), most notably in the N5 group, wherein reductions of 38.24% and 20.94% were observed, respectively. Additionally, parameters such as leaf area, leaf thickness, stem thickness, plant height, photosynthetic attributes, pigment content, soluble sugars, soluble proteins, proline, and the activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) were augmented to varying extents. Trends in the relative gene expression of SOD, POD, and CAT remained in accordance with their respective antioxidant enzyme activities. Correlation analysis further substantiated that leaf area and net photosynthetic rate (Pn) exerted substantial influence over SOD, POD, CAT activities, and their corresponding gene expression. In conclusion, the inoculation with nitrogen-fixing bacteria served to foster the growth and resilience of F. taipaiensis P. Y. Li, with the synergistic application of Rahnella aquatilis and Pseudomonas stellifer proving most efficacious. These insights not only offer a reference framework for selecting nitrogen-fixing bacteria as fertilizer in the subsequent artificial cultivation of F. taipaiensis P. Y. Li but also highlight the potential application value of these bacterial combinations.
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