PJB-2024-334
Protein profiling and proximate analysis of Chenopodium quinoa varieties adapted to Pakistan
Ansa Rani, Muhammad Yasin Ashraf, Ismat Umar, Ahmed Akrem, Sohaib Mehmood and Binish Khaliq
Abstract
Quinoa (Chenopodium quinoa Willd.) is a seed crop native to the Andean region, known for its ability to grow in diverse agro-ecological conditions and its high nutritional value. Its seeds contain significant amounts of various micro- and macronutrients that are beneficial for both animal and human health. In the current study, protein profiling, quantification and proximate chemical composition were evaluated for food purposes. Five quinoa varieties (BLK, Q-8, Q-8/39, Q-11 and Q-13) selected for their favorable agronomic traits, were analyzed for biochemical composition and quality characteristics. The highest protein concentration (21.5 mg/g, 15.2 mg/g) was found in seed extract of varieties Q-8 and BLK at 25°C and 4°C, respectively. SDS-PAGE profiling of reduced seed extract displayed protein with molecular weight up to 150 kDa and 50 kDa at 25°C and 4°C, respectively. In non-reduced samples, the highest molecular weight observed was 70 kDa and 60 kDa at 25°C and 4°C, respectively. The highest values for protein content (13.9%), crude fiber (1.3%), crude fat (3.3%), ash content (1.95%), moisture content (10.5%) and carbohydrate content (66.5%) were observed across different varieties. The maximum carbohydrates content (65.9%) was found in Q-13, while BLK exhibited the lowest crude fiber content (1.1%) among the varieties studied. These findings provide valuable insights for the development of high-quality quinoa-based products
To Cite this article:
Rani, A., M.Y. Ashraf, I. Umar, A. Akrem, S. Mehmood and B. Khaliq. 2025. Protein profiling and proximate analysis of Chenopodium quinoa varieties adapted to Pakistan. Pak. J. Bot., 57(6): DOI: http://dx.doi.org/10.30848/PJB2025-6(32)
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