PJB-2024-334
PROTEIN PROFILING AND PROXIMATE ANALYSIS OF CHENOPODIUM QUINOA VARIETIES ADAPTED TO PAKISTAN
ANSA RANI
Abstract
Quinoa (Chenopodium quinoa Willd) is the Andean region seed crop. It can grow in different agro-ecological conditions and have high nutritive value. Its seeds contain different micro- and macronutrients in large amount which are beneficial for animal and human health.. In current study, protein profiling and quantification were performed and proximate chemical composition were evaluated for food purpose. In this investigation five Quinoa varieties (BLK, Q-8, Q-8/39, Q-11, Q-13) with favorable agronomic parameters were analyzed for their biochemical composition and quality traits. The highest protein concentration (21.5 mg/g, 15.2 mg/g) was found in seed extract of variety Q-8 and BLK at 25°C and 4°C, respectively. Twelve percent SDS-PAGE profiling of reduced seed extract, displayed protein with molecular weight up to 150 kDa and 50 kDa at 25°C and 4°C respectively. In non-reduced samples, the highest molecular weight of protein was 70 kDa and 60 kDa at 25 °C and 4°C respectively. The greatest amount of protein content (13.9%), crude fiber (1.3%), crude fat (3.3%), ash content (1.95%), moisture content (10.5%) and carbohydrate content (66.5%) were recorded in seed extract of different varieties studied in the present research. Carbohydrates were found maximum in Q-13(65.9%), whereas, the minimum amount of crude fiber was recorded in BLK (1.1%) as compared to other Quina varieties. These outcomes would be beneficial to synthesize quality products from the quinoa.
Key words: Chenopodium quinoa, SDS-PAGE, chemical composition, Protein
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