PJB-2024-485
IDENTIFICATION OF ABIOTIC STRESS TOLERANT GENE-ORTHOLOGS OF BARLEY (HORDEUM VULGARE L.) USING ARABIDOPSIS: AN IN SILICO APPROACH
Muhammad Naveed Shahid
Abstract
Abiotic stresses have a detrimental effect on agricultural growth and yield globally. Barley (Hordeum vulgare) is grown on almost all continents because of its tolerance to a wide range of abiotic stresses. The current study finds gene orthologs responsible for salt-stress tolerance in barley. It seems possible to identify orthologs in other plants using the available information. For this purpose, 89 genes were selected from the scientific literature that had been peer-reviewed. To find the genes associated with abiotic stress, the Arabidopsis thaliana genome-wide gene expression database was examined. A total of 519 A. thaliana genes associated with salt-stress were shown using the STRING database and co-expression analysis. Fourteen clusters connected to 138 genes were discovered through cluster analysis. TAIR database was used to obtain the amino acid sequences of the Arabidopsis gene. Comparative analyses between the non-redundant Hordeum vulgare protein sequence and the Arabidopsis network-associated protein sequence identified 14 transcription factors and 9 A. thaliana domains with unclear functions. In comparison, barley had five domains with unknown functions and 9 transcription factors. The enrichment tool helped us to recognize six molecular functions that were crucial to the salt-stress response of plants. The orthologs data may be used to increase productivity and reduce the loss of barley yield on account of abiotic stresses.
To Cite this article:
Under Review
We Welcome Latest Research Articles In Field Of Botany The Pakistan Journal of Botany is an international journal .... Read More
