PJB-2024-499
REVEALING THE POTENTIAL OF AZADIRACHTA INDICA A. JUSS. FOR THE INDUCTION OF AMYLOLYTIC AND PULLULYTIC ENZYMES
SAIFULLAH*
Abstract
In vitro culture of Azadirachta indica offers controlled and constant provision of metabolites and therefore could be more feasible in enzyme induction study. In this research, we developed suspension cultures by neem callogenesis and incorporated different percentages of inducers, namely starch, dextrin and pullulan in the suspension media to analyse their effects on the induction of amylolytic and pullulytic enzymes. The cell free extracts of the induced cultures were detected on starch and pullulan agar-solidified plates for the zones of hydrolysis. Moreover, we estimated the enzyme activities by DNS method. Consequently, both starch and pullulan degrading activities were detected in the cultured media, indicating the induction of amylolytic and pullulytic enzymes. These enzymes are of great commercial importance and find extensive usage in food, textile and detergent industries. This is the first report in literature on pullulan degradation by neem enzymes and will provide great assistance to future scientists for further research in this area.
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