PJB-2024-526
Revealing the Chemical Composition via GC-MS Analysis and Its Mechanistic Effects on Diarrhea, Inflammation, and Pain from Curio radicans (L. f.) P.V. Heath.
Muhammad Naseer
Abstract
Abstract
Phytochemicals play a key role in the medicinal properties of plants, including anti-inflammatory, analgesic, and antidiarrheal effects. This study aimed to analyze the ethanolic and ethyle acetate extract of Curio radicans (L. f.) P.V. Heath through gas chromatography-mass spectrometry (GC-MS) analysis and evaluate its analgesic, anti-inflammatory, and antispasmodic activities. Bioactive compounds were identified by comparing their mass spectra with the National Institute of Standards and Technology (NIST) library. Analgesic effects were assessed using the hot plate (HP) and acetic acid-induced writhing (AAI) methods. The anti-inflammatory potential was tested using the carrageenan-induced paw edema model (PEM), while the gastrointestinal motility test (CMT) evaluated antispasmodic effects. GC-MS analysis of both ethanolic and ethyl acetate extracts revealed several bioactive compounds, these included pharmacologically active compounds such as Hexanedioic acid, cis-Vaccenic acid, 1,3-Benzenedicarboxylic acid, Glycerin, 2-Methoxy-4-vinylphenol, Propanal, and 2-propenylhydrazone. The AAI method showed significant peripheral analgesic activity (P<0.001) at 300mg/kg, while the HP method revealed increased basal reaction time (P<0.001) after 90 minutes. Anti-inflammatory testing demonstrated a reduction in paw volume (51.93%) at the same dose, and the CMT showed significant inhibition of charcoal movement, with the ethyl acetate extract inhibiting it by 51.03% at 300mg/kg. Compounds such as Hexanedioic acid, cis-Vaccenic acid, bis(2-ethylhexyl), 1,3-Benzenedicarboxylic acid, Glycerin, 2-Methoxy-4-vinylphenol, Propanal, and 2-propenylhydrazone may contribute to the bioactivity of the plant. The study demonstrated notable medicinal and therapeutic potential, which is considered novel. However, additional research is necessary to explore the underlying mechanisms responsible for these effects.
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