PJB-2024-533
THE IMPACT OF THE ADVENTITIOUS ROOT NUMBER ON THE GROWTH OF TISSUE-CULTURED BOMBAX CEIBA PLANTLETS
DEJIN MU
Abstract
Adventitious roots occur naturally in many species and can also be induced from explants of some tree species including Bombax ceiba L., providing an important means of clonal propagation. However, the contribution of adventitious roots to the subsequent growth of tissue-cultured B. ceiba remains largely unexplored. In this study, tissue-cultured plantlets of B. ceiba with 1 (One AR), 2 (Two ARs), and 3 adventitious roots (Three ARs) were taken as the test materials to compare differences in root morphology, drought resistance, and seedling quality among the three types of tissue-cultured plantlets. The results showed that the total root length and total root surface area of the three types of tissue-cultured plantlets differed between groups after 80 d of culture on the half-strength MS medium, while the differences in biomass, root volume, and total root mean diameter were not significant. In addition, tissue-cultured plantlets with three adventitious roots showed the strongest resistance to drought stress under different concentrations of polyethylene glycol (PEG-6000). Finally, seedling quality for three types of plantlets transplanted for 180 d was evaluated, which showed that, although one ARs plant tended to produce main roots more readily than the other types, three ARs plants tended to produce the highest levels of biomass and seedling quality. Therefore, this directly indicates that the number of adventitious roots will affect the quality of tissue-cultured plantlets in the later stage, providing a new insight for cultivating high-quality seedlings.
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