PJB-2024-541
COMPLETE CHLOROPLAST GENOME OF PREMNA OBTUSIFOLIA, A SEMI-MANGROVE PLANT
Shi-Quan Wang
Abstract
Premna obtusifolia is one of distributed semi-mangrove species in the world. In this research, whole chloroplast (cp) genome of P. obtusifolia was assembled for the first time. Meanwhile, this research expanded the chloroplast genome resource bank of semi-mangrove species. Comprehensive analysis about cp genome of P. obtusifolia showed prospective cpDNA markers, which could be used to study species identification, species adaptation mechanisms and phylogenetic status. The total cp genome was 153,415 bp in length, displaying paradigmatic tetrad structure, which included a large single copy (LSC) region of 84,649bp and a small single copy (SSC) region of 17,410bp, and they were segregated by two inverted repeats (IRs) regions of 25,678bp. Overall GC content was 38.02% of cp genome, and GC content in IRs, LSC, and SSC regions was 43.21%, 36.03%, and 32.38%, respectively. The genome was predicted through 114 unique genes, embodying 80 protein-coding genes, 30 tRNA genes, and 4 rRNA genes, and there were 19 duplicates in IR regions. 87 SSRs and 17 long repeats were recognized totally. Phylogenetic analysis suggested that P. obtusifolia was clustered with P. vietnamensis within genus Premna. This research shows that the cp genome of P. obtusifolia is conservative in structure with slow evolutionary rate and is more structurally differentiated compared with other species in premna. These results indicate that semi-mangrove species respond to the disturbance of the external environment well in the evolutionary history, and their genome characteristics have been preserved.
To Cite this article:
Download
