PJB-2024-544
DEVELOPMENT OF INVITRO REGENERATION PROTOCOL FOR OLIVE PLANTS
Ihsan Ul Haq
Abstract
A study entitled “Development of invitro regeneration protocol for olive plants” was conducted at Plant Tissue Culture Laboratory, Agricultural Research Institute (ARI) Tarnab, Peshawar during 2023-2024. The study was carried out in 5 steps, each one containing ten treatments (different media). All the experiments were carried out according to Complete Randomized Design (CRD), replicated three times. For statistical analysis of studied parameters, Statistical Software Statistic 8.1 was used. Differences among means were tested through LSD test. Young leaves from two years old Olive tree plant (cv Gemlik) were used as experimental material for the study. Results showed that on control media (without PGRs) callus induction did not accord, while minimum days to Callusing (15 days) and maximum callusing response (75%) were recorded in explants cultured on Murashige and Skoog medium (MS) supplemented with 2 mg l-1 Indole-3-acetic acid (IAA) + 1 mg l-1 kinetin (Kin). Early shoot initiation (20 days), maximum shooting frequency (80 %) and more number of shoots (3.7) per callus was achieved on Olive medium (OM) supplemented with 2.5 mg L-1 Kin + 0.5 mg l-1 Zeatin (Zin). Maximum Shoot elongation (5.3 cm) occurred on OM media supplemented 1 mg l-1 benzyl adenine BA + 0.1 mg l-1 Gibberellic acid (GA3). Maximum rooting response (84 %), number of roots (5.4), and root length (4.4 cm) was recorded on half strength MS media supplemented with 1.0 mg l-1 Indole-3-butyric acid (IBA). The regenerated plantlets were acclimatized successfully in controlled Greenhouse condition. Overall process was completed in 08 months; the developed protocol may be a useful tool for mass propagation of this valuable temperate fruit species. More research is needed to improve shooting and acclimatization protocol for this economical important plant specie.
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