PJB-2024-545
EFFECT OF VARIOUS GROWTH MEDIA ON MULTIPLE SHOOT PRODUCTION FROM OLIVE CALLI
Abubakar Sadeeq
Abstract
A study entitled “Effect of various growth media on multiple shoot production from Olive calli” was conducted at Plant Tissue Culture Laboratory, Agricultural Research Institute (ARI) Tarnab, Peshawar during 2023-2024. All the experiments were carried out according to Randomized Complete Design (CRD), replicated three times. For statistical analysis of studied parameters, Statistical Software Statistix 8.1 was used. Differences among means were tested through LSD test. Stem segments (10mm) of current year growth from two years old Olive tree plant (cv Airlik) were used as experimental material for the study. Results showed that on MS (Murashige and Skoog) basal media which is also called control media (without PGRs) callus induction did not accord, while minimum days to Callusing (18 days) and maximum callusing response (80%) were recorded in explants cultured on MS media supplemented with 2 mg L-1 IAA (Indole-3-acetic acid) + 1 mg L-1 Kn (kinetin). Maximum shooting frequency (85 %) and more number of shoots (5.2) per callus was achieved on Olive medium (OM) supplemented with 2.5 mg L-1 Kn + 0.5 mg L-1 Zn (Zeatin). Result shows that MS solid media supplemented with 2 mg L-1 IAA + 1 mg L-1 Kn was optimal for early and maximum callus induction from stem segments explant. While OM media supplemented with 2.5 mg L-1 Kn + 0.5 mg L-1 Zn was effective for maximum shooting frequency and more number of shoots from calli. More research is needed to improve shooting, rooting and acclimatization protocol for this economical important plant specie.
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