PJB-2024-554
Molecular Identification and Characterization of Chilli Veinal Mottle Virus (Potyvirus) incidence in tomato and other Solanaceae crops
Abdulrahman Alasmari
Abstract
Potyviruses are the largest plant infecting and cause of heavy yield loss worldwide. Chilli Veinal Mottle Virus (ChiVMV) mainly infests Solanaceae crops. In this present study, we are collecting infected tomato plants such as mottling, chlorotic patches from East & West Tabuk, Kingdom of Saudi Arabia and used for molecular analysis, and host range studies. The DAC-ELISA results led to confirmation of ChiVMV in these samples. RT-PCR was performed and ChiVMV- CP genes were amplified, cloned, and sequenced, based on the NCBI-BLASTn. The virus infected samples were mechanically inoculated on to tomato seedlings. Mosaic, mottling and leaf distortion symptoms were observed in tomato seedlings and these symptoms were similar to observed in tomato field from where samples collected originally, thus fulfilling the Koch’s postulate. The RT-qPCR results showed that the presence of ChiVMV of about 1337000 and 6911000 folds compared to healthy plants in East Tabuk and Wast Tabuk isolates respectively. ChiVMV infecting tomato has not been reported earlier, this is the first report that infects tomatoes and its host surveys and symptomological characteristics and molecular characterization of ChiVMV isolates. It shows that ChiVMV not only infects chilli, but it also infects tomatoes, and the spread of this virus may affect the yields of tomato crops in Tabuk. This was the preliminary study, and it needs brief distribution for ChiVMV tomato cultivations.
To Cite this article:
Download
