PJB-2025-1517
Growth media from plant extracts for industrially important fungus; Aspergillus niger
Sadiah Saleem, Tasneem Adam Ali and Sana Eijaz
Abstract
The current study is performed to assess growth supporting capability of aqueous plant-based extracts which were further supplemented with sugarcane peel extract; SPEX and mineral salt solution (MSS) to formulate microbiological growth media for fungi particularly Aspergillus niger. Dry cell weight (DCW), extracellular proteins, reducing sugars, pH alteration in extract media (EM) was observed and compared with that of potato dextrose broth (PDB). The production of citric acid and enzymes (amylase/cellulase; endogluconase) were also assessed on preferred EM combinations. Highest DCW was found in coconut bark extract + SPEX + MSS; CBSM, Nimtree Bark + SPEX; NBS and date bark extract + SPEX + MSS; DBSM (0.207, 0.16 and 0.2 gram/ 25 ml) respectively with SPEX 30 % (v/v) in comparison to that of PDB (0.155 gram/ 25 ml) on day 4. The pH of EM was acidic with 2.81; the lowest in NBS with SPEX 30% (v/v). Reducing sugars were detectable in EM containing SPEX 30 and 20% (v/v) concentration. Protein content was maximum in NBS, DBSM and CBSM (33, 24.75 and 22.44 µg/ml) respectively with various SPEX concentrations on day 4 as compared to that in PDB (20.75 µg/ml). NBS with 30% (v/v) SPEX showed highest concentration of citric acid (7.33 g/l) whereas, amylase/endoglucanase activity was better in DBEX + MSS (0.2 and 0.0283 IU /ml) on day 3 and 7 respectively. It can be concluded from the above results that the EM combinations have been found to be suitable for growth and other activities of Aspergillus niger.
To Cite this article:
Saleem, S., T.A. Ali and S. Eijaz. 2025. Growth media from plant extracts for industrially important fungus; Aspergillus niger. Pak. J. Bot., 57(3): DOI: http://dx.doi.org/10.30848/PJB2025-3(2)
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