PJB-2025-1518
Regulatory effects of different growth habitats on the microbial composition and chemicals produced in K326 tobacco
Shen Huang, Ke Wang, Mengyue Chen, Aamir Rasool, Robina Manzoor, Duobin Mao, Jizhong Wu and Changhe Cheng
Abstract
K326 has excellent botanical and economic properties, good smoke appearance quality, orange color, moderate and strong gloss, medium and more oil content, loose leaf structure, moderate and thin leaf thickness, suitable nitrogen to base ratio, and high industrial availability. The quality of flue-cured tobacco depends on the types of chemicals produced in its leaves. In one tobacco plant, the total amount of furans, aldehydes and organic acids in middle tobacco leaves was significantly higher than that in upper tobacco leaves, but there was no significant difference between middle tobacco leaves and lower tobacco leaves. The total amount of phenols and organic acids in the pyrolysis products of the lower tobacco leaves was the highest and significantly higher than that of the upper tobacco leaves, but the difference was not significant compared with the middle tobacco leaves. There was significant negative correlation between total alkaloids, total nitrogen, total sugars and reducing sugars in all three parts. The different habitats effectively regulate the production of chemicals in tobacco by controlling the composition of the microbiome of its leaves. Nicotine content is different in different growing environments, for example, altitude and nicotine content are negatively correlated. Therefore, the effect of seven different habitats, including Sichuan, Hunan, Yunnan, Henan, Fujian, and Guizhou, on the composition of the K326 tobacco microbiome was investigated in this study using high throughput 16S rRNA Illumina MiSeq sequencing technology in this study. The results demonstrated that the abovementioned growth habitats strongly regulated the composition of the microbiome of K326 tobacco, which resultantly affected the production of chemicals. The following bacterial genera, such as Allorhizobium, Neorhizobium, Pararhizobium, and Rhizobium, displayed significantly positive correlations with the potassium and nitrogen-nicotine contents of K326 tobacco leaves. Methylobacterium influenced the production of 5-Methylfuranaldehyde and furfural. Halomonas positively correlated with the production of key neutral aroma compounds (NACs) in K326 tobacco leaves, such as geraniol, 4-Oxyisofluorone,Phenyl ethanol and Damascusone. Our findings show that the aroma of K326 tobacco can be engineered by controlling the microbiome composition by growing tobacco in selective habitats
To Cite this article:
Huang, S., K. Wang, M. Chen, A. Rasool, R. Manzoor, D. Mao, J. Wu and C. Cheng. 2025. Regulatory effects of different growth habitats on the microbial composition and chemicals produced in K326 tobacco. Pak. J. Bot., 57(3): DOI: http://dx.doi.org/10.30848/PJB2025-3(3)
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