Paper Details

PJB-2025-23

Formulation and characterization of novel Bacillus subtilis loaded starch nanoparticles against fungal pathogens

Ayesha Khan and Sadia Javed
Abstract

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The aim of the current study was to synthesize and characterize the Bacillus subtilis-loaded starch nanoparticles against fungal pathogens. The starch nanobeads were synthesized via ultra-sonication and Bacillus subtilis was subsequently encapsulated onto the beads. The Bacillus subtilis loaded starch nanoparticles were characterized by XRD and SEM to confirm the crystalline structure and spherical shape of nanobeads. Bacillus subtilis loaded starch nanoparticles showed a Z-average size of 245 nm and zeta potential of −20.25 (mV) as indicated by zeta potentials. FTIR analysis demonstrated the interactions between starch nanobeads and Bacillus subtilus. The antifungal potential was tested against Fusarium oxysporum, Fusarium graminearum, Aspergillus flavus, Alternaria solani, Botrytis cinerea, Verticllium dahlia, and Cladosporium cucumirinum. A clear inhibition zone and minimum inhibition concentration (MIC) was observed that indicates the significant antifungal effect of the Bacillus subtilis loaded starch nanoparticles. Functional effectiveness of Bacillus subtilis starch nanoparticles for practical uses could be among the primary concerns of future research

To Cite this article: Khan, A. and S. Javed. 2026. Formulation and characterization of novel Bacillus subtilis loaded starch nanoparticles against fungal pathogens. Pak. J. Bot., 58(2): DOI: http://dx.doi.org/10.30848/PJB2026-2(14)  
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