PJB-2025-47
A PRELIMINARY STUDY ON THE IN VITRO OVULE CULTURE OF TREE PEONY
HAOANG MA
Abstract
Despite the extensive history of breeding and propagation, precious hybrid tree peony varieties still suffer from low seed setting and germination rates. In vitro ovule culture has emerged as a promising approach to mitigate embryo abortion and facilitate the germination of seed embryos into seedlings. In this study, we initially explored the effects of embryo age, seed coat presence, medium type, concentration of culture medium and light environment on the in vitro culture of 'Fengdan' ovules across various embryonic stages. Subsequently, we conducted a comparative analysis of the 80 days old ovules of 'Fengdan', 'Riyuejin', 'Liantianjin', and 'Baiyuan Hongxia'. Results indicated that the ovules of 'Fengdan' can only develop under appropriate in vitro culture conditions. Specifically, peeled ovules, when inoculated in a solid medium and with an age of over 70 days, were viable, whereas browning was observed in liquid suspension culture. Moreover, the embryo germination rate was higher in 1/2MS medium and under a higher light intensity of 2000 Lx. Across all varieties examined, an inverse correlation was evident between the browning rate and the embryo germination rate. This relationship is likely associated with the embryonic ages, more precisely, when the embryo materials in vitro culture have reached a definite extent of organ differentiation, the browning rate decreases, while the embryo germination rate increases.
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