PJB-2025-60
Biosynthesis and Characterization of zinc oxide nanoparticles using aqueous leaves extract of Kalanchoe pinnata; from antimicrobial to biomedical applications
Sadia Sheraz
Abstract
Kalanchoe pinnata mediated synthesis of zinc oxide nanoparticles (ZnONPs) using zinc acetate as the zinc source was successfully prepared. Today, nanotechnology has become one of the developing areas in the preparation of nanoparticles by biological procedures. Extraction from plant is a source of bioactive compounds and it is one of the most effective biological synthesis methods. In the UV-Vis spectrum, ZnONPs were identified to exhibit a surface plasmon resonance peak at 347 nm. FTIR analysis revealed that a functional biomolecules become linked to the ZnONPs. This confirmed by the particle size and SEM analyses which demonstrate that ZnONPs were in the range of 50–120 nm. The particles of ZnONPs were analyzed using EDX measured in the range of 0 to 14 keV. The crystalline form of the synthesized ZnONPs was confirmed by X-ray diffraction. The characterized ZnONPs were employed in diverse biological activities such as antibacterial, antifungal, antioxidant, analgesic, anti-inflammatory, and antipyretic assays. The bio-fabricated nanoparticles were proved to have greater efficacy as analgesic and free radical scavenging activity than the plant extracts alone. The microbial resistance of ZnONPs was highest by antifungal activity against Aspergillus niger accompanied by antibacterial assessment on some human pathogenic strains such as Escherichia coli. The green synthesized ZnONPs have shown significant anti-oxidant, anti-inflammatory, analgesic and antipyretic activity.
Keywords: Kalanchoe pinnata, Zinc oxide, nanoparticles, biological applications.
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