PJB-2025-1533
Phytochemical profiling of wild pomegranate (Punica granatum L.): unveiling antioxidant potential and antibacterial activity
Jamil Ullah, Israr Ahmad, Mohammad Nisar, Irfan Ullah, Asif Khan, Yong-Sun Moon, Muhammad Siraj, Alaa S. Alhegaili, Sajid Ali and Muhammad Hamayun
Abstract
Wild Pomegranates Punica granatum L., is a medicinal plant, native to Northern Pakistan and is known for its richer phytochemicals profile and bioactive properties. In this study, we used HPLC to analyze the phytochemical composition and assess the antioxidant and antibacterial activities of different parts of the plants. Interestingly, the seed extract exhibited the highest antioxidant activity, with IC50 values of 795 µg/mL for DPPH and 975 µg/mL for ABTS. Among the tested parts, rind extract showed the highest bacterial growth inhibition against Xanthomonas oryzae and the effect may be attributed to its high phenolic contents. Similarly, HPLC characterization of methanolic extract of different parts of wild pomegranate identified various phytochemicals, including malic acid, vitamin C, chlorogenic acid, quercetin, ellagic acid, epigallocatechin gallate and pyrogallol. However, specifically, the rind contained five phytochemicals including, malic acid, vitamin C, quercetin, chlorogenic acid and pyrogallol. The root contained six phytochemicals: malic acid, vitamin C, epigallocatechin gallate, quercetin, chlorogenic acid, and ellagic acid. Similarly, the seed contained six phytochemicals: malic acid, vitamin C, epigallocatechin gallate, quercetin, chlorogenic acid and ellagic acid. It was concluded that wild pomegranate is a rich source of beneficial phytochemicals, with the seed extract displaying potent antioxidant properties and the rind extract showing strong antibacterial activity. Further In vivo and toxicological studies are necessary to validate these findings
To Cite this article:
Ullah, J., I. Ahmad, M. Nisar, I. Ullah, A. Khan, Y.S. Moon, M. Siraj, A.S. Alhegaili, S. Ali and M. Hamayun. 2025. Phytochemical profiling of wild pomegranate (Punica granatum L.): unveiling antioxidant potential and antibacterial activity. Pak. J. Bot., 57(4): DOI: http://dx.doi.org/10.30848/PJB2025-4(29)
Download PDF
We Welcome Latest Research Articles In Field Of Botany The Pakistan Journal of Botany is an international journal .... Read More
