Paper Details

PJB-2025-192

Establishment of a regeneration system from seed explants of Saururus chinensis

Xue Yang, Xiao-Qian Xiong, Wan-Yun Wu, Zhan-Nan Yang and Tian-Hua Yu
Abstract

---

Saururus chinensis is as a relic plant and its seed germination is notably slow and inefficient under natural conditions. To find a fast propagation method, a regeneration system of S. chinensis seedlings is established utilizing seeds as explant, employing plant tissue culture technique. The results showed that the optimal disinfection protocol was determined to be 75% ethanol treatment for 30 s followed by 0.1% (w/v) HgCl₂ treatment for 10 min. This treatment resulted in a sterile germination rate of 37.41% and a contamination rate of only 5.56%. Furthermore, soaking seeds in 1.00 mg/L GA₃ for 2 hours and supplementing the culture medium with 0.20 mg/L IBA significantly improved germination, reaching a rate of 52.22%. During the seedling growth stage, the optimal medium for seedling development was identified as MS supplemented with 0.20 mg/L NAA+0.50 mg/L GA₃+1.00 mg/L IBA, under these conditions, seedlings reached an average height of 3.43 cm, developed dense root systems, and exhibited an average root length of 1.17 cm. To promote growth vigor, the subculture medium was further optimized to 0.50 mg/L NAA, 2.00 mg/L GA₃ and 0.20 mg/L IBA; this optimization produced seedlings with broad, dark green leaves, a plant height of 4.13 cm, dense root systems, and root lengths of 1.32 cm. Finally, regenerated seedlings were acclimatized in sterile soil and successfully transplanted to natural soil conditions, achieving a survival rate of 88%. Our work effectively supports the aseptic propagation and large-scale production, germplasm conservation, artificial breeding, and biological research of S. chinensis.

 

To Cite this article: Yang, X., X.Q. Xiong, W.Y. Wu, Z.N. Yang and T.H. Yu. 2026. Establishment of a regeneration system from seed explants of Saururus chinensis. Pak. J. Bot., 58(4): DOI: http://dx.doi.org/10.30848/PJB2026-4(18)  
Download PDF