PJB-1992-14
PLANT-REGENERATION IN MESOCOTYL CALLUS OF BRASSICA-JUNCEA VAR POORBIRAYA
SHAHZADI N; AHMAD Z; HUSSAIN A
Abstract
Mesocotyl segments of Brassica juncea var. poorbiraya from in vitro grown seedlings were tested for their callogenic and thence morphogenic potential with various treatments of plant growth regulators. Calli grown at 1.0 mg/l of BAP gave a maximum frequencey(2.25 buds/culture) of shoot regeneration. Regenerated buds were induced to root when cultured on half strength MS medium containing IAA @ 0.5 mg/l. Embryogenesis was induced in mesocotyl callus in MS medium containing 2,4-D (0.022 mg/l) + KIN (0.215 mg/l). Phenotype of regenerated plants looked similar to in vitro grown Brassica juncea plants.
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