PJB-2007-278
IN VITRO CLONAL PROPAGATION AND BIOCHEMICAL ANALYSIS OF FIELD ESTABLISHED STEVIA REBAUDIANA BERTONI
MUHAMMAD RAFIQ1*, MUHAMMAD UMAR DAHOT1, SHER MUHAMAMD MANGRIO2, HABIB AHMED NAQVI1 AND IQBAL AHMED QARSHI3
Abstract
An experiment was conducted to optimize growth medium and growth conditions for In vitro propagation of Stevia rebaudiana Bertoni through nodular stem sections. Varying concentrations of different growth regulators BAP, Kn, and IAA were applied through growth medium to assess their effects on shoots development while NAA and IBA were applied to assess their role in root formation. Although all plant growth regulators promoted shoot formation, maximum shoot formation was observed by supplementing 2.0 mgL-1 BAP. In contrast 0.5 mgL-1 NAA caused the maximum root formation in nodular stem sections of S. rebaudiana. Furthermore, survival rate of regenerated plants were 92 and 83% during hardening and shifting to green house, respectively. Various biochemical attributes (carbohydrates, proteins) in the leaves were also measured and compared with leaf extract of Egyptian cultivar to assess its quality.
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