PJB-2007-310
BIOSYNTHESIS OF ACID PROTEASES BY PENICILLIUM GRISEOROSEUM IH-02 IN SOLID-STATE FERMENTATION
IKRAM-UL-HAQ AND HAMID MUKHTAR
Abstract
The present investigation describes the production of acid proteases by Penicillium griseoroseum IH-02 using solid-state fermentation technique. The enzyme biosynthesis was maximum (8.2 U/ml) when fermentation was carried out on a substrate containing soybean meal and wheat bran mixed at a ratio of 3:1 and moistened with D4 diluent. The substrate to diluent ratio was found to be optimum at 1:2. The extraction of the enzyme from fermented broth was carried out with different extractant and distilled water having pH 5.0 was found to be the best extractant of the enzyme.
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