PJB-2008-115
GAMMA IRRADIATION EFFECTS ON SEED GERMINATION AND GROWTH, PROTEIN CONTENT, PEROXIDASE AND PROTEASE ACTIVITY, LIPID PEROXIDATION IN DESI AND KABULI CHICKPEA
AMJAD HAMEED, TARIQ MAHMUD SHAH, BABAR MANZOOR ATTA, M. AHSANUL HAQ AND HINA SAYED
Abstract
Desi and kabuli chickpea seeds irradiated with 100 to 1000 Gy gamma rays (with an interval of hundred) were grown in incubator for 8 days at 25 °C. Germination, growth (seedling fresh weight, root shoot length and ratio), lipid peroxidation, protease and peroxidase activity were measured in leaves. Results showed that percent germination of the seeds and the rates of growth of sprouts were inversely related to the irradiation doses. In kabuli chickpea, peroxidase and protease activities (two folds) and MDA contents were higher as compared to desi chickpea while vice versa for protein contents, revealing inherent differences between two types. Data for protein contents, peroxidase and protease activities therefore suggested that irradiation dose should not exceed 600Gy in kabuli chickpea and 500Gy in desi chickpea. In kabuli chickpea 500Gy irradiation dose non-significantly affected the protein contents and peroxidase activity and lowered MDA contents and protease activity. In desi chickpea 400Gy irradiation dose increased the peroxidase activity, lowered the MDA contents and did not affect the protein content and protease activity. It was concluded that protein contents, protease, peroxidase and lipid peroxidation may be used in early assessment of effectiveness and superiority of radiation dose to induce mutations.
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