Paper Details

PJB-2008-139

ISOLATION OF A XYLAN DEGRADING GENE FROM GENOMIC DNA LIBRARY OF A THERMOPHILIC FUNGUS CHAETOMIUM THERMOPHILE ATCC 28076

FOUZIA SALEEM1, SIBTAIN AHMED1,2 AND AMER JAMIL1*
Abstract

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The most abundant hemicellulosic polymers xylans, constitutes about 20-40% of total plant biomass. Xylanases are involved in fruit softening, seed germination and plant defense systems. In this study, genomic DNA of Chaetomium thermophile ATCC 28076 was isolated and a genomic library was created in E.coli DH10B-pUC 19 host vector system. Positive clones were screened by Congo red staining. The E.coli harboring xylanase gene showed clear zone with Congo red clearance assay on xylan plates.

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