PJB-2008-178
NEW APOPTOTIC EFFECT OF D-MANNOSE IN WHEAT ROOTS
AMJAD HAMEED1 , NAYYER IQBAL1 AND SALMAN A. MALIK2
Abstract
Effect of D-mannose on antioxidant defense response, apoptotic internucleosomal nuclear DNA (nDNA) degradation, and protease activity was studied in roots of wheat seedlings during early growth. Mannose (1%) induced apoptotic internucleosomal nDNA fragmentation after 96 hours of treatment when it was not observed in control. Superoxide dismutase activity was down regulated parallel to induction of apoptosis. Protease activity was enhanced prior to induction of apoptosis which shows involvement of proteolysis in apoptotic induction process. More over a sharp increase in membrane lipid peroxidation (MDA content) was also observed during apoptosis induction. Catalase and peroxidase activities were increased 24 hours before apoptotic induction while down regulated at time of induction. Total phenolic content was down regulated 48 hours before induction of apoptosis. Collectivity antioxidant defense was down regulated and proteases activities and lipid peroxidation were enhanced during induction of apoptosis and thus play a key role in controlling D-mannose induced apoptotic process. New apoptotic effect of D-mannose in wheat roots in connection with antioxidants and proteases activities is discussed in detail.
To Cite this article:
Download
