PJB-1996-8
INHERITANCE OF RAPD MARKERS IN THE F1 INTERSPECIFIC HYBRIDS OF RICE
S. FAROOQ, T.M. SHAH, M. ARIF AND N. IQBAL
Abstract
Random amplified polymorphic DNA markers were generated in a diploid and two tetraploid salt-tolerani accessions of wild rice species Oryza punctata, O. sativa cuitivars Basmati - 198, IR-6 and F1 hybrids between Basmati-198 and IR-6 with three accessions of O. punctata used as male parent. The PCR mediated DNA amplification profiles produced with different 10 mer primers were generally reproducible and differendsled tetraploid accessions from diploid and the hybrids from their parents. Of the total 263 markers that were generated with II different primers, 164 markers (62 %) were exhibited by 5 parents and 102 markers (38 %) by 3 different F1 hybrid combinations. In 2 of the 3 hybrids, frequency of the markers inherited from O. punctata was above 70%, as most of the primers amplified in the hybrid, DNA fragments inherited from O. punctata (male parent). Most of the markers transferred from the male parent to the hybrids were of the same intensity while those transferred from the female parent (IR-6 and Basmati-198) were of low intensity compared to their intensity in the female parent itself. The detection of the male and female parent specific fragments in the hybrids was primer dependent. Our study indicated the reproducibility and reliability of the RAPD markers in identification of wild and cultivated rice varieties and their F1 hybrids. The significance of RAPD markers in monitoring specific characters transferred from the wild parents to the cultivated rice varieties is discussed.
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