PJB-2009-311
EFFECT OF DIFFERENT CULTURAL CONDITIONS ON MICROPROPAGATION OF ROSE (ROSA INDICA L.)
ASAD SHABBIR1, NOSHEEN HAMEED1 AMIR ALI2 AND RUKHSANA BAJWA1
Abstract
The aim of the present investigation was to determine a appropriate basal medium and growth regulators for In vitro propagation of Rosa indica from shoot apical meristems. The shoot meristem was cultured on MS medium supplemented with different concentrations of BAP ranging from 0.5–2.5 mg/l alone and in combination with 0.5 mg/l of kinetin for shoot formation and multiplication. Medium containing 1.5 mg/l BAP proved to be the best medium for In vitro shoot formation from apical meristem. At this concentration, rate of shoot formation was 94% obtained in 6.2 days. Maximum 20 shoots per culture were formed on medium having 1.0 mg/l of BAP + Kinetin. This medium proved to be the best for In vitro shoot multiplication and proliferation of roses.
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