PJB-2010-290
BIOCHEMICAL INVESTIGATION DURING DIFFERENT STAGES OF IN VITRO PROPAGATION OF STEVIA REBAUDIANA
AAMIR ALI1*, IRUM GULL2, SHAGUFTA NAZ3 AND SHAHID AFGHAN4
Abstract
In vitro propagation of Stevia rebaudiana Bert, an important non-caloric sweetening herb was carried out to explore its potential for micropropagation (both from apical and nodal meristem) and callogenesis (using leaf, node and internode as explant). MS basal medium with 1.0 mg l-1 of BAP was found to be the best medium for shoot formation, with 90% shoot formation response within 12 days of meristem inoculation, both from shoot apical and nodal meristem. Maximum shoot multiplication response (90%) was also obtained in MS medium having 1.0 mg l-1 of BAP, with average of 8.6 shoots per culture vial having an average shoot length of 6.0 cm. The best In vitro rooting response (96%) was recorded on MS medium containing 1.0 mg l-1 NAA within 7.3 days of inoculation. When well developed In vitro plants were shifted for hardening on a mixture of sand + soil + peat (1:1:1) 90% success was recorded. For callogenesis leaf explant proved to be the best followed by nodal and internodal explant. The highest response of callus induction from leaf explant was obtained on MS medium supplemented with 3.0 mg l-1 2,4-D while nodal and internodal explants showed best results for callogenesis in MS medium supplemented with 3.0 mg l-1 NAA and 1.0 mg l-1 BAP. The highest total soluble protein contents and the peroxidases activity were estimated in the six week old callus cultures derived from leaf explant.
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