PJB-2010-301
MOLECULAR AND EPIDEMIOLOGICAL ANALYSIS OF MULTIDRUG-RESISTANT SALMONELLA PARATYPHI A OBTAINED FROM DIFFERENT REGIONS OF PAKISTAN
ZEBA PARWEEN IMRAN1, ANITA ZAIDI2, ANWER ALI SIDDIQUI2, SATHEESH NAIR3 AND AQEEL AHMAD*4
Abstract
Incidence of paratyphoid infections caused by Salmonella paratyphi A is significantly mounting high in the country. Indiscriminate use of antibiotics which often results in the development of drug-resistant strains, is making the treatment difficult. This situation becomes worst when organism acquire multidrug resistance (MDR). In the present study, various isolates of MDR S. paratyphi A, obtained from different regions of Pakistan, were analyzed for plasmid profile and multiplex PCR to determine the resistance gene associated with plasmid and chromosomal DNA of S. paratyphi A. Multi-drug resistant isolates of S. paratyphi A obtained during the year 2001-2006 from different regions of Pakistan have been included in this study. These isolates showed resistance to chloramphenical (30µg), ampicillin (10µg), trimethoprim-sulfamethoxazole (23.5µg), and tetracycline (30µg). Some strains were also found resistant to ofloxacin and nalidixic acid. The plasmids from bacterial strains were found to be approximately 220 kbp, harboring antibiotic resistance genes. A multiplex PCR was used to identify any variation in antibiotic resistance genes encoding the MDR phenotypes in clinical isolates of S. paratyphi A. A homogenous pattern of multiplex PCR product revealed that MDR isolates of S. paratyphi A harbored the same resistance genes. This study will help taking effective measures for controlling disease in the region, and the data could be used for future medical reference.
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