PJB-2010-303
MYCOFLORA ASSOCIATED WITH SUGAR CANE JUICE IN KARACHI CITY
ALMAS AHMED, SHAHNAZ DAWAR AND MARIUM TARIQ
Abstract
The study was conducted for investigating the quality of 28 sugar cane juice samples collected from different localities of Karachi. pH value of sugarcane juice samples with lemon ranged from 4.60-6.56, whereas the sugarcane juice samples without lemon ranged from 5.0-6.85. Mycoflora of samples was studied by using direct plate and serial dilution techniques. Twenty eight samples of sugarcane juice with and without lemon were tested and 18 different species belonging to 11 different genera of fungi isolated by direct plating method were Absidia corymbifera, Acremonium sp., Aspergillus flavus, A. fumigatus, A. niger, A. sulphureus, A. terreus, A. wentii, Fusarium semitectum, F. sporotrichoides, Humicola grisea, Gilmanieila humicola, Curvularia lunata, Monilia sp., Rhizopus stolonifer, R. oryzae, Penicillium sp., and yeast (Saccharomyces spp.) whereas Aspergillus candidus, A. subolivaceous, A. erythrocephalus and A. tamarii were isolated in addition to these by serial dilution techniques. The highest number of fungi were isolated by serial dilution technique and A. niger appeared as a dominant fungus of sugarcane juice with and without lemon by both of the techniques. The addition of lemon juice reduced the occurrence of A. corymbifera, C. lunata and A. erythrocephalus by serial dilution technique.
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