PJB-2011-225
AN ATTEMPT TO CONSERVE WITHANIA SOMNIFERA (L.) DUNAL - A HIGHLY ESSENTIAL MEDICINAL PLANT, THROUGH IN VITRO CALLUS CULTURE
JYOTI RANJAN ROUT*, SANTI LATA SAHOO AND RITARANI DAS
Abstract
A simple effective protocol was developed for conservation and plant propagation through callus cultures of Withania somnifera (Ashwagandha). Seed germination percentage reached a maximum value of 64.3% on ½ MS + GA3 0.25 mg/l at third week of culture. Three different basal media compared for seed germination, MS was most effective. Out of 25 combinations of growth regulators evaluated, MS + 1.0 mg/l BA + 1.0 mg/l 2, 4-D found to be best for callus induction and proliferation regardless to explants. Among the four different explants tested, In vivo leaf explant was found most suitable for callus induction, proliferation and fresh weight gain. The highest callus induction frequency percentage 86.4% was recorded with In vivo leaf explant whereas, 43.4% in In vitro leaf explant at day 30 on MS augmented with 1.0 mg/l BA + 1.0 mg/l 2,4-D. Among different growth regulator combinations tested in augmentation with MS for shoot initiation and elongation
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