PJB-2011-255
IN VITRO GERMINATION OF HYBRID EMBRYOS FROM INTERPLOIDAL CROSSES OF CITRUS
ASIM MEHMOOD1, MUHAMMAD JAFAR JASKANI1*, YASAR SAJJAD1, IFTIKHAR AHMAD2 AND ZAHOOR HUSSAIN3
Abstract
Interploid crosses between Feutrell’s early (2x), Kinnow mandarin (2x), Succari sweet orange (2x) and Kinnow (4x) were made for the production of triploid hybrids. Maximum fruit set (100%) was observed between crosses of Kinnow (2x) X Kinnow (4x) and maximum June drop (95.5%) was noted when diploid Succari as female parent was crossed with tetraploid Kinnow. The hybrid previous termfruitsnext term harvested after 12–14 weeks or 7 months after pollination produced many underdeveloped and few developed previous termseeds;next term however, tetraploid Kinnow as previous termseednext term parent yielded more developed previous termseedsnext term per previous termfruit. Immature previous termembryos of seedsnext term harvested 12–14 weeks after pollination were cultured In vitro on MS medium supplemented with adenine sulphate (0.25 g l−1), malt extract (0.5 g l−1) and sucrose (50 g l−1). Maximum previous termgerminationnext term (58.6%) of hybrid previous termembryosnext term from developed previous termseedsnext term was obtained in 4x X 2xprevious termcross of Kinnow strains.
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